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Special sample collection |
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(Dutch elm Disease, Oak Wilt, Verticillium Wilt) |
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Collect 3 small branch pieces, 1/2 inch in diameter, 4 to 6 inches long, from recently wilted branches. Pieces from the living sections of branches that are partially alive and partially dead are best. do not send branches that have dried out and have a brown layer directly under the bark. |
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Collect small soil samples from several locations in a field to be tested for nematodes, pea/bean root rot pathogens or other soil-borne pathogens. Try to collect a sample that represents the soil from all areas of the field. These small samples (subsamples) can be mixed together for shipment.
For nematode, Verticillium, and Aphanomyces/Pythium/Phytophthora non-specific testing submit 1-2 cups of soil.
For Aphanomyces race-specific testing, submit 2-3 gallons of soil per sample. |
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Cut plugs that are 3 to 4 inches in diameter from the edge of the affected area where healthy turf meets diseased turf. |
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Call the PDDC when planning to submit a sample for bacterial ring rot testing to verify there will be a clinician available to perform the test in a timely manner.
An appropriate sample consists of cores cut from the stem ends of 400 (+/- 8) tubers (one core per tuber). If the number of cores is outside this range, the sample will be rejected. Cores should be approximately 1 cm deep by 1 cm wide, should be cut in a roughly conical shape, and should include as much of the vascular tissue at the stem attachment site as is possible within these size limitations (see diagram). If cores are too large and contain too much non-vascular tissue, it can compromise the sensitivity of test results.
The tubers from which the cores are cut should be a representative sample of a field or lot, not, for example, just the top tubers in the bin. If the tuber sample is not representative of the field or lot, the accuracy of detection of bacterial ring rot will be decreased.
Place each lot of cores in clean, plastic bags that are clearly labeled and sealed well. Double bagging of cores is preferred.
Time the sampling of tubers to allow shipping as soon as possible after sampling. Store samples in a refrigerator between sampling and shipping. Verify the integrity of the plastic bag(s) before shipment, this is especially important to prevent leakage if several samples are shipped together.
Pack samples with ice packs to keep them cool during shipping. Add additional packing material to the shipping container to cushion the sample.
Enclose submission information for each sample and protect it from moisture by placing it in a sealed plastic bag. Include information such as: submitter's name, mailing address, telephone number, email address and fax number (optional), date of collection, date of shipment, and a description of each lot of cores in the shipment (field, lot, and variety numbers/names).
Seal the shipping box or container well to protect sample integrity. Use a mail carrier that will deliver sample to the laboratory on a Monday, Tuesday, Wednesday or Thursday. Overnight shipping is highly recommended. |
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Select a representative group of leaves (or other plant parts) that exhibit the range of symptoms that you have observed in the field.
Be sure to collect detailed information on the location where the sample was collected so that the site can be revisited if necessary. If you know the GPS coordinates of the site, please provide these.
If possible, place the leaves between layers of cardboard and paper towels to keep them flat (i.e., layer the materials as follows - cardboard, paper towel, leaves, paper towel, cardboard, paper towel, leaves, paper towel, cardboard, etc.).
Place the leaves in a self-sealing plastic bag and seal the bag shut.
Place this bag inside a second self-sealing plastic bag, being particularly careful that the outside of this second bag does not become contaminated.
Keep the leaves cool (e.g., by placing them in a cooler or refrigerator) between the time of collection and the time when they are shipped to the PDDC.
Ship the samples to the PDDC by overnight mail whenever possible. |
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