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Jo Handelsman
(608) 263-8783
Fax (608) 265-5289

Department of Plant Pathology
University of Wisconsin - Madison
1630 Linden Dr.
Madison, WI 53706

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Prof. Jo Handelsman

Jo Handelsman
Ph.D.: University of Wisconsin-Madison in Molecular Biology

Research Program

The Handelsman Lab

Research in the Handelsman lab focuses on understanding the structure and function of microbial communities, addressing questions that have challenged plant pathologists and microbiologists since the inception of our discipline. We are finding ways to better describe the organization of microbial communities through decoding the language by which the members communicate. This understanding will lead to development of new technologies to enhance plant health and discovery of new medicinal and agricultural chemistry.

The emerging image of microbial communication networks is one of numerous bacterial signals that are often scrambled or amplified by other members of the community. At this early stage in our understanding it is difficult to imagine how bacteria make sense of their chemical environment as they are bombarded with signals from all sides. But the study of carefully chosen systems will reveal the networks that provide order to the communication, and understanding communication, in turn, will help elucidate the structure and function of microbial communities. We have selected three discrete but complementary systems for this phase of our work, one of which follows the activities of a specific bacterium in a community context, one that explores the challenges of defining community, and one that represents a contained ecosystem that can be manipulated in the laboratory.

  • We are examining the interaction of Bacillus cereus with its host plant and the microbial community in which it functions, allowing for a detailed modeling of the chemical signaling in the plant rhizosphere that determines bacterial survival, gene expression, and effects on the host plant and on protists that affect plant health. [MORE-1]
  • We are using a new methodology, termed 'metagenomics,' to examine the genomes of uncultured microorganisms in soil communities, offering a fantastic and largely unexplored complement to our current cultu re-based understanding of microbial life that will bring new definition to the concept of microbial community. [MORE-2]
  • We are using the gypsy moth midgut as a wholly contained ecosystem, asking questions about the role of each of the microorganisms within the microbial community and as factors affecting the physiology of the host. [MORE-3]

Students and Staff

Click here for current students and staff, or click here for alumni of the Handelsman Lab.

Wisconsin Program For Scientific Teaching Staff: Sarah Miller (Co-Director); Chris Pfund (Co-Director); Sandra Gossens (Administrative Specialist); Christina Matta (Project Assistant); Rodolfo John Alaniz (Program Assistant)

On November 12, 2005, the Handelsman Lab hosted an inter-lab meeting, "It Takes A Village: Cooperation Between Animals and their Microbial Communities." Click here to see images from the meeting.

Teaching sites

Plant Path / Botany 123 - Plants, Parasites, and People

The Spud Files

Biology Brought To Life: A Guidebook To Teaching Students To Think Like Scientists

Chemical Methods

Turbomycin Chemical Methods (pdf format)
Turbomycin Chemical Methods (MS-Word format)

Statistical Methods

S-LIBSHUFF

DOTUR

Books

Kleinman, D.L., Matta, C., Cloud-Hansen, K., Handelsman, J. (eds.) 2007. Controversies in Science and Technology, Volume II: From Chromosomes to the Cosmos. Mary Ann Liebert, Inc.

Handelsman, J., Miller, S., Pfund, C. 2006. Scientific Teaching. W. H. Freeman and Company, New York , NY.

Handelsman, J., Pfund, C., Miller Lauffer, S., and Pribbenow, C.M. 2005. Entering Mentoring: A Seminar to Train a New Generation of Scientists. University of Wisconsin Press, Madison, WI.

Kleinman, D.L., Kinchy, A.J., and Handelsman, J., eds. 2005. Controversies in science and technology: From maize to menopause. University of Wisconsin Press.

Handelsman, J., Houser, B.J., and Kriegel, H. 1997. Biology Brought to Life: A guide to teaching students how to think like scientists. Wm. C. Brown Publishers, Inc., Dubuque, Iowa.

Handelsman, J. 1997. Biology Brought to Life: Laboratory Guidebook. Wm. C. Brown Publishers, Inc., Dubuque, Iowa.


Education and Women in Science

Sheridan , J., Flatley-Brennan, P., Carnes, M., Handelsman, J. 2006. Discovering directions for change in higher education through the experiences of senior women faculty. Journal of Technology Transfer. 31(3):387.

Pfund, C., Pribbenow, C.M., Branchaw, J., Miller Lauffer, S., and Handelsman, J., 2006. The merits of training mentors. Science. 311(5760): 473-474. PDF Available.

Handelsman, J., Cantor, N., Carnes, M., Denton, D., Fine, E., Grosz, B., Hinshaw, V., Marrett, C., Rosser, S., Shalala, D., Sheridan, J. 2005. More women in science. Science: 309:1190-1191. PDF Available. Supplemental material also available (PDF).

Carnes, M., Geller, S., Fine, E., Sheridan, J., and Handelsman, J. 2005. NIH Director's Pioneer Awards: Could the selection process be biased against women? Journal of Women's Health 14(8): 682-689. PDF Available.

Carnes, M., Handelsman, J., and Sheridan, J. 2005. Diversity in academic medicine: The stages of change model. 2005. Journal of Women's Health 14(6): 471-475. PDF Available.

Handelsman, J., and Wood, W.B. 2004. Meeting report: The 2004 National Academies Summer Institute on Undergraduate Education in Biology. Cell Biol Education 3: 215-217. PDF Available

Handelsman, J., Ebert-May, D., Beichner, R., Bruns, P., Chang, A., DeHaan, R., Gentile, J., Lauffer, S., Stewart, J., Tilghman, S.M., Wood, W.B. 2004. Scientific Teaching. Science 304: 521-522. PDF Available. Supplemental information also available (PDF).

Lauffer, S. and Handelsman, J. 2004. A new generation of scientific teachers.Focus on Microbiology Education 10:4-6.

Handelsman, J. 2003. Teaching scientists to teach. HHMI Bulletin 12: 31. PDF Available.

Handelsman, J. 2002. Microbiology as a change agent in science education. ASM News 68:163-167. PDF Available.

Review Articles and Editorials

Scholss, P.D., Handelsman J. 2007. The last word: books as a statistical metaphor for microbial communities. Annu Rev Microbiol. 2007. 61:23-34. Online Access Available.

Cloud-Hansen, K.A., Peterson, S.B., Stabb, E.V., Goldman, W.E., McFall-Ngai, M.J., and Handelsman, J. 2006. Breaching the great wall: peptidoglycan and microbial interactions. Nature Rev. Microbiol. 4:710-716. PDF Available.

Matta, C., and Handelsman, J. 2006. It takes a village: Role of indigenous microbial communities in infectious disease. In: Ending the War Metaphor. National Academies Press, Washington, D.C. PDF Available.

Handelsman, J. 2006. Metagenomics or megagenomics? Nature Rev. Microbiol. 3: 457-458. PDF Available.

Gillespie, D., Rondon, M.R., Williamson, L.L., and Handelsman, J. 2005. Metagenomic libraries from uncultured microorganisms. In: Molecular Microbial Ecology. A.M. Osborn and C.J. Smith, eds. Bios Sci. Pub., New York. pp. 261-279. PDF Available.

Handelsman, J. 2005. How to find new antibiotics. The Scientist. 19: 20. PDF Available.

Handelsman, J., Robinson, C.J., and Raffa, K. 2005. Microbial communities in lepidopteran guts: from models to metagenomics. In: The Influence of Cooperative Bacteria on Animal Host Biology. M.J. McFall-Ngai, B. Henderson, and E.G. Ruby, eds. Cambridge University Press, New York. pp. 143-168. PDF Available.

Schloss, P.D., and Handelsman, J. 2005. Metagenomics for studying unculturable microorganisms: Cutting the Gordian Knot. Genome Biology 6: 229.1-229.4. PDF Available.

Handelsman, J. 2005. Sorting out metagenomes. Nature Biotech. 23: 38-39. PDF Available.

Handelsman, J. 2004. Metagenomics: Application of genomics to uncultured microorganisms. Microbiol. Molec. Biol. Rev. 68: 669-685. PDF Available.

Riesenfeld, C., Schloss, P.D., and Handelsman, J. 2004. Metagenomics: genomic analysis of microbial communities. Annual Review of Genetics 38: 525-52. PDF Available.

Handelsman, J. 2004. Soils - the metagenomics approach. Pages 109-120 in: Microbial Diversity and Biosprospecting. ASM Press. PDF Available.

Handelsman, J., and Smalla, K. 2003. Conversations with the silent majority. Current Op. Micro. 6:271-273. PDF Available.

Schloss, P.D., and Handelsman, J. 2003. Biotechnological prospects from metagenomics. Current Op. Biotech. 14:303-310. PDF Available.

Handelsman, J., Liles, M., Mann, D., Riesenfeld, C., and Goodman, R.M. 2002. Cloning the metagenome: culture-independent access to the diversity and functions of the uncultivated microbial world. Pages 241-255 in: Methods in Microbiology - Functional Microbial Genomics. Academic Press. PDF Available.

Dunn, A.K., and Handelsman, J. 2002. Toward an understanding of microbial communities through analysis of communication networks. ISME 9 Antonie von Leewenhoek 81: 565-574. PDF Available.
Handelsman, J. and Wackett, L. 2002. Ecology and industrial microbiology: Microbial diversity - sustaining the Earth and industry. Current Op. Microbiol. 5:237-239. PDF Available.

Handelsman, J. 2002. Future trends in biocontrol. In: Biological Control of Crop Diseases. S.S. Gnanamanickam, ed. Marcel Dekker, Inc., New York. pp. 443-448. PDF Available.

Rondon, M.R., Goodman, R.M., and Handelsman, J. 1999. The Earth's bounty: assessing and accessing soil microbial diversity. Trends in Biotechnology 17:403-409. PDF Available.

Handelsman, J., Rondon, M.R., Brady, S., Clardy, J., and Goodman, R.M. 1998. Molecular biology provides access to the chemistry of unknown soil microbes: a new frontier for natural products. Chemistry & Biology 5:R245-R249. PDF Available.

Stohl, E.A., Stabb, E.V., and Handelsman, J. 1996. Zwittermicin A and biological control of oomycete pathogens. In: Biology of plant-microbe interactions. G. Stacey, B. Mullin, and P.M. Gresshoff, eds. St. Paul. pp. 475-480.

Handelsman, J., and Stabb, E.V. 1996. Biocontrol of soilborne plant pathogens. Plant Cell 8:1855-1869. PDF Available.

O'Connell, K.P., Goodman, R.M., and Handelsman, J. 1996. Engineering the rhizosphere: Expressing a bias. TibTech 14:83-88. PDF Available.

Research Papers

Mark R. Liles, Lynn L. Williamson, Jitsupang Rodbumrer, Vigdis Torsvik, Robert M. Goodman, and Jo Handelsman. 2008. Recovery, Purification, and Cloning of High-Molecular-Weight DNA from Soil Microorganisms. Appl. Environ. Microbiol. 74(10): 3302-3305 PDF Available

Isenbargar, T. A., Finney, M., Rios-Velazquez, C., Handelsman, J., and Ruvkun, G. 2008. Miniprimer PCR, a New Lens for Viewing the Microbial World. Appl. Environ. Microbiol. 74(3): 840-849 PDF Available

Vasanthakumar, A., Handelsman, J., Schloss, P.D., and Raffa, K.F. Gut microbiota of an invasive wood boring beetle, the emerald ash borer: community composition and structure across different life stages. FEMS. (in review)

Schloss, P.D. and Handelsman, J. A Statistical Toolbox for Metagenomics: Assessing Functional Diversity in Microbial Communities. Genome Biology. (in review)

Delalibera, I. Vasanthakumar, A., Burwitz, B.J., Schloss, P.D., Klepzig, K.D., Handelsman, J. and Raffa, K.F. 2007. Composition of the bacterial community in the gut of the pine engraver, Ips pini (Say) (Coleoptera) colonizing Red Pine. Symbiosis. 43: 97-104. PDF Available.

Guan, C., Ju, J., Borlee, B.R., Williamson, L.L., Shen, B., Raffa, K.F., and Handelsman, J. 2007. Signal mimics derived from a metagenomic analysis of gypsy moth gut microbiota. Appl. Environ. Microbiol. 73(11): 3669-3676. PDF Available.

Vasanthakumar, A., Delalibera Jr. I., Handelsman, J., Klepzig, K.D., Schloss, P., and Raffa, K.F. 2006 Characterization of gut-associated microorganisms in larvae and adults of the southern pine beetle, Dendroctonus frontalis Zimmerman. Environ. Entomol. 35:1710-1717. PDF Available.

Schloss, P.D. and Handelsman, J. 2006. Toward a census of bacteria in soil. PLoS Computational Biology 2:e92. PDF Available.

Chan, Y.A., Boyne, M.T., Podevels, A.M., Klimowicz, A.K., Handelsman, J., Kelleher, N.L., and Thomas, M.G. 2006. Two new polyketide synthase extender units: hydroxymalonyl-acyl carrier protein and aminomalonyl-acyl carrier protein. Proc. Natl. Acad. Sci. USA. 103(41):14349-14354.

Peterson, S.B., Dunn, A.K., Klimowicz, A.K., and Handelsman, J. 2006. Peptidoglycan from Bacillus cereus mediates commensalism with rhizosphere bacteria from the Cytophaga-Flavobacterium group. App. Environ. Microbiol. 72:5421-5427.

Chan, Y.A., Boyne, M.T., Podevels, A.M., Klimowicz, A.K., Handelsman, J., Kelleher, N.L., and Thomas, M.G. 2006. Two new polyketide synthase extender units: hydroxymalonyl-acyl carrier protein and aminomalonyl-acyl carrier protein. Proc. Natl. Acad. Sci. USA. 103(41):14349-14354.

Broderick, N.A., Raffa, K.F., and Handelsman, J. Midgut bacteria required for Bacillus thuringiensis insecticidal activity. PNAS 103: 15196-15199. PDF Available.

Schloss, P.D., and Handelsman, J. 2006. Introducing SONS, a tool for operational taxonomic unit-based comparisons of microbial community memberships and structures. App. Environ. MIcrobiol 72:6773-6779. PDF Available.

Sabree, S.L., Bergendahl, V., Liles, M.R., Burgess, R.R., Goodman, R.M., Handelsman, J. 2006. Identification and characterization of the gene encoding the Acidobacterium capsulatum major sigma factor. Gene 376:144-151. PDF Available.

Schloss, P.D., and Handelsman, J. 2006. Introducing TreeClimber, a test to compare microbial community structures. Appl. Environ. Microbiol. 72: 2379-2384. PDF Available.

Schloss, P.D., Delalibera, I., Handelsman, J., and Raffa, K.F. 2006. Bacteria associated with the guts of two wood-boring beetles: Anolophora glabripennis and Saperda vestita (Cerambycidae). Environ. Entomol. 35: 625-629. PDF Available.

Williamson, L.L, Borlee, B.R., Schloss, P.D., Guan, C., Allen, H.K., and Handelsman, J. 2005. Intracellular screen to identify metagenomic clones that induce or inhibit a quorum-sensing biosensor. Appl. Environ. Microbiol. 71: 6335-6344. PDF Available.

Delalibera, I., Raffa, K., and Handelsman, J. 2005. Contrasts in cellulolytic activities of gut microorganisms between the wood borer, Saperda vestita (Coleoptera: Cerambycidae) and the bark beetles, Ips pini and Dendroctonus frontalis (Coleoptera: Curculionidae). Environ Entomol. 34: 541-547. PDF Available.

Schloss, P.D., and Handelsman, J. 2005. Introducing DOTUR, a computer program for defining operational taxonomic units and species richness. Appl. Environ. Microbiol. 71: 1501-1506. PDF Available.

Schloss, P.D., and Handelsman, J. 2004. Status of the microbial census. Microbiol. Molec. Biol. Rev. 68: 686-691. PDF Available.

Schloss, P.D., Larget, B., and Handelsman, J. 2004. Integration of microbial ecology and statistics: a test to compare gene libraries. Appl. Environ. Microbiol. 70: 5485-5492. PDF Available.

Broderick, N.A., Raffa, K., Goodman, R.M., and Handelsman, J. 2004. Census of the bacterial community of the gypsy moth larval midgut using culturing and culture-independent methods. Appl. Environ. Microbiol. 70: 293-300. PDF Available.

Safdar, N., Handelsman, J., and Maki, D.G. 2004. Does combination antimicrobial therapy reduce mortality in Gram-negative bacteraemia? A meta-analysis. The Lancet - Infectious Diseases. 4:519-27.

Liles, M.R., Williamson, L.L., Handelsman, J., and Goofman, R.M. 2004. Isolation of high molecular weight genomic DNA from soil bacteria for genomic library construction. In: Molecular Microbial Ecology Manual, 2nd ed. G.G. Kowalchuk, F.J. de Bruijn, I.M. Head, A.D. Akkermans, and J.D. van Elsas, eds. Kluwer Academic Publishers, The Netherlands. pp. 839-852.

Riesenfeld, C.S., Goodman, R.M., and Handelsman, J. 2004. Uncultured soil bacteria are a reservoir of new antibiotic resistance genes. Environ. Microbiol. 6:981-989. PDF Available.

Emmert, E.A.B., Klimowicz, A.K., Thomas, M.G., and Handelsman, J. 2004. Genetics of zwittermicin A production in Bacillus cereus. Appl. Environ. Microbiol. 70: 104-113. PDF Available.

Liles, M.R., Manske, B.F., Bintrim, S.B., Handelsman, J., and Goodman, R.M. 2003. A census of rRNA genes and linked genomic sequences within a soil metagenomic library. Appl. Environ. Microbiol. 69: 2684-2691. PDF Available.

Broderick, N.A., Goodman, R.M., Handelsman, J., and Raffa, K.F. 2003. Effect of host diet and insect source on synergy of gypsy moth (Lepidoptera:Lymantriidae) mortality to Bacillus thuringiensis subsp. kurstaki by zwittermicin A. Environ. Entomol. 32: 387-391. PDF Available.

Dunn, A.K., Klimowicz, A.K., and Handelsman, J. 2003. Use of a promoter trap to identify Bacillus cereus genes regulated by tomato seed exudate and a rhizosphere resident, Pseudomonas aureofaciens. Appl. Environ. Microbiol. 69: 1197-205. PDF Available.

Gillespie, D.E., Brady, S.F., Bettermann, A.D., Cianciotto, N.P., Liles, M.R., Rondon, M.R., Clardy, J., Goodman, R.M., and Handelsman, J. 2002. Isolation of antibiotics turbomycin A and B from a metagenomic library of soil microbial DNA. Appl. Environ. Microbiol. 68: 4301-4306. PDF Available.

Brady, S.F., Chao, C.J., Handelsman, J., and Clardy, J. 2001. Cloning and heterologous expression of a natural product biosynthetic gene cluster from eDNA. Organic Letters 3:1981-1984. PDF Available.

Simon, H.M., Smith, K.P., Dodsworth, J.A., Guenthner, B., Handelsman, J., and Goodman, R.M. 2001. Influence of tomato genotype on growth of inoculated and indigenous bacteria in the spermosphere. Appl. Environ. Microbiol. 67:514-520. PDF Available.

Bittinger, M.A., Gross, J.A., Widom, J., Clardy, J., and Handelsman, J. 2000. Rhizobium etli CE3 carries vir gene homologs on a self-transmissible plasmid. Molec. Plant-Microbe Interact. 13:1019-1021. PDF Available.

Kazmar, E.R., Goodman, R.M., Grau, C.R., Johnson, D.W., Nordheim, E.V., Undersander, D.J., and Handelsman, J. 2000. Regression analyses for evaluating the influence of Bacillus cereus on alfalfa yield under variable disease intensity. Phytopathology 90:657-665. PDF Available.

Rondon, M.R., August, P.R., Bettermann, A.D., Brady, S.F., Grossman, T.H., Liles, M.R., Loiacono, K.A., Lynch, B.A., MacNeil, I.A., Minor, C., Tiong, C.L., Gilman, M., Osburne, M.S., Clardy, J., Handelsman, J., and Goodman, R.M. 2000. Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms. Appl. Environ. Microbiol. 66:2541-2547. PDF Available.

Bittinger, M.A., and Handelsman, J. 2000. Identification of genes in the RosR regulon of Rhizobium etli. J. Bacteriol. 182:1706-1713.
PDF Available.

Broderick, N.A., Goodman, R.M., Raffa, K.F., and Handelsman, J. 2000. Synergy between zwittermicin A and Bacillus thuringiensis subsp. kurstaki against gypsy moth (Lepidoptera: Lymantriidae). Environ. Entomol. 29:101-107. PDF Available.

Stohl, E.A., Milner, J.L., and Handelsman, J. 1999. Zwittermicin A biosynthetic cluster. Gene 237:403-411. PDF Available.

Stohl, E.A., Brady, S.F., Clardy, J., and Handelsman, J. 1999. ZmaR, a novel and widespread antibiotic resistance determinant that acetylates zwittermicin A. J. Bacteriol. 181:5455-5460. PDF Available.

Raffel, S.J., Kazmar, E.R, Winberg, R., Oplinger, E.S., Handelsman, J., Goodman, R.M., and Grau, C.R. 1999. First report of root rot of soybeans caused by Corynespora cassiicola in Wisconsin. Plant Dis. 83:696. PDF Available.

Rondon, M.R., Raffel, S.J., Goodman, R.M., and Handelsman, J. 1999. Toward functional genomics in bacteria: Analysis of gene expression in Escherichia coli from a bacterial artificial chromosome library of Bacillus cereus. Proc. Natl. Acad. Sci. USA 96:6451-6455. PDF Available.

Smith, K.P., Handelsman, J., and Goodman, R.M. 1999. Genetic basis in plants for interactions with disease-suppressive bacteria. Proc. Natl. Acad. Sci. USA 96:4786-4790. PDF Available.

Shang, H., Chen, J.-J., Handelsman, J., and Goodman, R.M. 1999. Behavior of Pythium torulosum zoospores during their interaction with tobacco roots and Bacillus cereus. Current Microbiol. 38:199-204. PDF Available.

Dunn, A.K., and Handelsman, J. 1999. A vector for promoter trapping in Bacillus cereus. Gene 226:297-305.
PDF Available.

Emmert, E.A.B., and Handelsman, J. 1999. Biocontrol of plant disease: a (Gram-) positive perspective. FEMS Microbiology Letters 171:1-9. PDF Available.

Emmert, E.A.B., Milner, J.L., Lee, J.C., Pulvermacher, K.L., Olivares, H.A., Clardy, J., and Handelsman, J. 1998. Effect of canavanine from alfalfa seeds on the population biology of Bacillus cereus. Appl. Environ. Microbiol. 64:4683-4688. PDF Available.

Rosas, J.C., Castro, J.A., Robleto, E.A., and Handelsman, J. 1998. A method for screening Phaseolus vulgaris L. germplasm for preferential nodulation with a selected Rhizobium etli strain. Plant and Soil 203:71-78. PDF Available.

O'Connell, K.P., Raffel, S.J., Saville, B.J., and Handelsman, J. 1998. Mutants of Rhizobium tropici strain CIAT899 that do not induce chlorosis in plants. Microbiology 144:2607-2617. PDF Available.

Biermann, B.J., de Banzie, J.S., Handelsman, J., Thompson, J.F., and Madison, J.T. 1998. Methionine and sulfate increase a Bowman-Birk-type protease inhibitor and its messenger RNA in soybeans. J. Agric. Food Chem. 46:2858-2862. PDF Available.

Silo-Suh, L.A., Stabb, E.V., Raffel, S.J., and Handelsman, J. 1998. Target range of zwittermicin A, an aminopolyol antibiotic from Bacillus cereus. Current Microbiology 37:6-11. PDF Available.

Stabb, E.V., and Handelsman, J. 1998. Genetic analysis of zwittermicin A resistance in Escherichia coli: effects on membrane potential and RNA polymerase. Molecular Microbiology 27:311-322. PDF Available.

Smith, K.P., Handelsman, J., and Goodman, R.M. 1997. Modeling dose-response relationships in biological control: partitioning host responses to the pathogen and biocontrol agent. Phytopathology 87:720-729.

Bittinger, M.A., Milner, J.L., Saville, B.J., and Handelsman, J. 1997. rosR, a determinant of nodulation competitiveness in Rhizobium etli. Mol. Plant-Microbe Interact. 10:180-186.

Bintrim, S.B., Donohue, T.J., Handelsman, J., Roberts, G.P., and Goodman, R.M. 1997. Molecular phylogeny of Archaea from soil. Proc. Natl. Acad. Sci. 94:277-282. PDF Available.

Raffel, S.J., Stabb, E.V., Milner, J.L., and Handelsman, J. 1996. Genotypic and phenotypic analysis of zwittermicin A-producing strains of Bacillus cereus. Microbiology 142:3425-3436.

Milner, J.L., Silo-Suh, L., Lee, J.C., He, H., Clardy, J., and Handelsman, J. 1996. Production of kanosamine by Bacillus cereus UW85. Appl. Environ. Microbiol. 62:3061-3065. PDF Available.

Gilbert, G.S., Clayton, M.K., Handelsman, J., and Parke, J.L. 1996. Use of cluster and discriminant analyses to compare rhizosphere bacterial communities. Microbial Ecology 32:123-147.

Milner, J.L., Stohl, E.A., and Handelsman, J. 1996. Zwittermicin A resistance gene from Bacillus cereus. J. Bacteriol. 178:4266-4272.
PDF Available.

Chen, J., Jacobson, L.M., Handelsman, J., and Goodman, R.M. 1996. Compatibility of systemic acquired resistance and microbial biocontrol for suppression of plant disease in a laboratory assay. Molecular Ecology 5:73-80.

Milner, J.L., Raffel, S.J., Lethbridge, B.J., and Handelsman, J. 1995. Culture conditions that influence accumulation of zwittermicin A by Bacillus cereus UW85. Applied Microbiol. Biotechnol. 43:685-691.

Osburn, R.M., Milner, J.L., Oplinger, E.S., Smith, R.S., and Handelsman, J. 1995. Effect of Bacillus cereus UW85 on the yield of soybean at two field sites in Wisconsin. Plant Dis. 79:551-556.

Stabb, E.V., Jacobson, L., and Handelsman, J. 1994. Zwittermicin A-producing strains of Bacillus cereus from diverse soils. Appl. Environ. Microbiol. 60:4404-4412.

Silo-Suh, L.A., Lethbridge, B.J., Raffel, S.J., He, H., Clardy, J., and Handelsman, J. 1994. Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85. Appl. Environ. Microbiol. 60:2023-2030.

Araujo, R.S., Robleto, E.A., and Handelsman, J. 1994. A hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere. Appl. Environ. Microbiol. 60:1430-1436.

He, H., Silo-Suh, L.A., Handelsman, J., and Clardy, J. 1994. Zwittermicin A, an antifungal and plant protection agent from Bacillus cereus. Tetrahedron Letters 35:2499-2502. PDF Available.

Gilbert, G.S., Handelsman, J., and Parke, J.L. 1994. Root camouflage and disease control. Phytopathology 84:222-225.

O'Connell, K.P., and Handelsman, J. 1993. Foliar chlorosis in symbiotic host and non-host plants induced by Rhizobium tropici type B strains. Appl. Environ. Microbiol. 59:2184-2189.

Halverson, L.J., Clayton, M.K., and Handelsman, J. 1993. Variable stability of antibiotic resistance markers in Bacillus cereus UW85 in the soybean rhizosphere in the field. Molecular Ecology 2:65-78.

Halverson, L.J., Clayton, M.K., and Handelsman, J. 1993. Population biology of Bacillus cereus UW85 in the rhizosphere of field-grown soybeans. Soil Biol. Biochem. 25:485-493.

Beattie, G.A., and Handelsman, J. 1993. Evaluation of a strategy for identifying nodulation competitiveness genes in Rhizobium leguminosarum biovar phaseoli. J. Gen. Microbiol. 139:529-538. PDF Available.

Smith, K.P., Havey, M.J., and Handelsman, J. 1993. Suppression of cottony leak of cucumber with Bacillus cereus strain UW85. Plant Dis. 77:139-142.

Gilbert, G.S., Parke, J.L., Clayton, M.K., and Handelsman, J. 1993. Effects of an introduced bacterium on bacterial communities on roots. Ecology 74:840-854.

Milner, J.L., Araujo, R.S., and Handelsman, J. 1992. Molecular and symbiotic characterization of exopolysaccharide-deficient mutants of Rhizobium tropici strain CIAT899. Molecular Microbiol. 6:3137-3147.

Halverson, L.J., and Handelsman, J. 1991. Enhancement of soybean nodulation by Bacillus cereus UW85 in the field and in a growth chamber. Appl. Environ. Microbiol. 57:2767-2770.

Handelsman, J., Nesmith, W.S., and Raffel, S.J. 1991. Microassay for biological and chemical control of infection of tobacco by Phytophthora parasitica var. nicotianae. Current Microbiol. 22:317-319. PDF Available.

O'Connell, K.P., Araujo, R.S., and Handelsman, J. 1990. Exopolysaccharide deficient mutants of Rhizobium strain CIAT899 induce chlorosis in beans. Molec. Plant-Microbe Interact. 3:424-428.

Handelsman, J., Raffel, S., and Sequeira, L. 1990. Monoclonal antibodies against Agrobacterium tumefaciens strain C58. Current Microbiol. 21:181-185.

Handelsman, J., Raffel, S.J., Mester, E.H., Wunderlich, L., and Grau, C.R. 1990. Biological control of damping-off of alfalfa seedlings by Bacillus cereus UW85. Appl. Environ. Microbiol. 56:713-718.

Gilbert, G.S., Handelsman, J., and Parke, J.L. 1990. Role of ammonia and calcium in lysis of zoospores of Phytophthora cactorum by Bacillus cereus UW85. Exper. Mycol. 14:1-8.

Beattie, G., Clayton, M., and Handelsman, J. 1989. Quantitative comparison of Rhizobium leguminosarum bv. phaseoli competitiveness in the laboratory and the field. Appl. Environ. Microbiol. 55:2755-2761.

O'Connell, K.P., and Handelsman, J. 1989. chvA locus may be involved in export of neutral B-1,2-linked D-glucan from Agrobacterium tumefaciens. Molec. Plant-Microbe Interact. 2:11-16.

Beattie, G.A., and Handelsman, J. 1989. Rapid method for identification of nodule bacteria. J. Microbiol. Methods 9:29-33.

Handelsman, J., Mester, E.H., Wunderlich, L., and Grau, C. 1988. Rapid screening of bacteria for biocontrol of Phytophthora damping off of alfalfa. Biol. Cult. Tests 3:60.

Ugalde, R.A., Handelsman, J., and Brill, W.J. 1986. Role of galactosyltransferase in competitiveness of Rhizobium meliloti. J. Bacteriol. 166:148-154.

Handelsman, J., and Brill, W.J. 1985. Erwinia herbicola isolated from alfalfa roots may play a role in nodulation by Rhizobium meliloti. Appl. Environ. Microbiol. 49:818-821.

Handelsman, J., Ugalde, R.A., and Brill, W.J. 1984. Rhizobium meliloti competitiveness and the alfalfa agglutinin. J. Bacteriol. 157:703-707.